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IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.
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| Test: |
Measles Virus Antibody, IgM |
| Synonym: |
Measles Virus Serology, Rubeola Antibody |
| Method: |
CDC capture IgM, Commercial indirect IgG |
| Availability: |
Monday - Friday. Turn-around time 2-7 days |
| Specimen: |
Blood |
| Collection Device: |
One 6.0 mL SST (Gold) tube or one 6.0 mL Clot (Red) tube |
| Volume: |
0.5 - 2.0 mL serum |
| Storage/Transport: |
Separate serum ASAP and transfer to clean tube. Refrigerate at 2-8°C. Sender should contact the NPHL by email or phone before shipping. Ship specimen Monday -Thursday overnight to avoid weekend deliveries. Refrigerated specimen should be shipped on cold packs. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address at http://nphl.org/. |
| Unacceptable: |
Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles, specimens stored at <-40°C |
| Specimen Stability: |
After separation from cells: ambient, < 8 hours; refrigerated (2-8°C), 72 hours; frozen (≥ -40°C), 1 year |
| Reference Interval: |
NEGATIVE - No significant level of detectable IgM antibody to measles virus.
EQUIVOCAL - Borderline levels of IgM antibody to measles virus. (Repeat testing in 10-14 days recommended).
POSITIVE - IgM antibody to measles virus detected |
| Reportable Disease: |
A positive result is reportable |
| Comments: |
IgM positive may not occur until 4 days post-rash onset. While the presence of IgM antibodies suggests current or recent measles infection, low levels of IgM antibodies may occasionally persist for > 12 months post-infection or immunization. Serum specimens WILL NOT be accepted for serology testing without the simultaneous submission of a specimen appropriate for Measles PCR. Test performed at CDC.
Pre-Approval needed from state or local health department. Send approval to NPHL@unmc.edu or call (402) 559-9444. |
| Revised: |
2/24/2015 |
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If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.
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