Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Shigella Serotyping  
  Synonym: Shigella typing, serogrouping 
  Method: Latex Agglutination  
  Availability: Test performed Daily, results within 24 - 48 hours.  
  Specimen: Pure isolate on media slant or plate  
  Collection Device: Organisms isolated from routine cultures submitted on media slant
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Not applicable  
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address:  
  Unacceptable: Mixed cultures  
  Specimen Stability: Stable at Ambient 15-25°C  
  Reference Interval: Negative  
  Reportable Disease: Positive results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send approval to or call (402) 559-9444.
Supplemental Forms Required – Nulirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.

Test Includes serogrouping and banking of referred organism. Include the test method used for identification on the request form. 
  Revised: 7/15/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.