Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Chikungunya Virus RNA Detection  
  Synonym: Arbovirus, Chikungunya virus RT-PCR  
  Method: RT-Polymerase Chain Reaction (PCR)  
  Specimen: Serum and cerebrospinal fluid (CSF)  
  Collection Device: 
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  

Prior to and during shipment:  Frozen on Dry Ice.
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Ship in insulated box on dry ice using UN1845 label. Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address:
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles. Heparinized specimens.  
  Specimen Stability: 2-8°C for 72 h, if > 3 d, freeze  
  Reference Interval: NEGATIVE - No significant level of detectable chikungunya virus RNA
POSITIVE – Chikungunya virus RNA detected  
  Reportable Disease:  A positive result is reportable as Arbovirus infection under Title 173, report within 7 days  
  Comments: Pre-approval needed from the state or local health department. Send approval to or call (402) 559-9444.

Test Request Order Form must accompany the specimen to include the following information:  symptoms and date of onset, pregnancy status, travel and vaccine history.  
  Revised: 3/15/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.