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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Chikungunya Virus Antibodies, IgM  
  Synonym: Arbovirus, Chikungunya virus serology  
  Method: ELISA  
  Availability: Test performed Monday-Friday ; Turn-around time 7 days  
  Collection Device:  

Centrifuge and aliquot serum into sterile leak-proof tube, ASAP. Place both CSF and serum at 4°C.
Shipping instructions, including specimen handling requirements during transport: Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped on cold packs Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.    
See instructions and shipping address:  Do not send directly to CDC.

  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles.  
  Specimen Stability: After separation from cells: Ambient 2-8°C for 48h; Refrigerated Refrigerate 2-8°C for 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
  Reference Interval: Negative - No significant level of detectable chikungunya virus IgM antibody.
Equivocal - Questionable presence of antibodies.  Repeat testing in 10-14 days may be helpful.
Positive –IgM antibody to chikungunya virus detected, which may indicate a current or past infection.  
  Reportable Disease: A positive result is reportable as Arbovirus infection under Title 173, report within 7 days  
  Comments: Pre-approval needed from the state or local health department.  Send approval to or call (402) 559-9444.
Test Request Order Form must accompany the specimen to include the following information:  symptoms and date of onset, pregnancy status, travel and vaccine history.  
  Revised: 3/15/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.