Syphilis IgG
Order in NUlirt or complete NPHL Test Order Form


Treponema pallidum IgG


Multiplex Flow Immunoassay


Test performed Monday – Friday when received by 0800; results reported same day


Whole blood

  Collection Device:

One 6mL SST (Gold tube) or Red tube.

Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.


2mL serum; 0.5mL minimum.


Prior to and during shipment: Refrigerate 2-8°C; If transport to lab is not the same day as collected, aliquot the serum into sterile tube. 

Shipping instructions, including specimen-handling requirements during transport:  Place in biohazard bag with absorbent material. 

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.


Plasma or other body fluids, severely lipemic, contaminated or hemolyzed specimens.

  Specimen Stability:

After separation from cells: Ambient: <8 hr; Refrigerated: up to 7d; For longer storage, freeze at -20 º Celsius.  Avoid repeat freeze/thaw cycles.

  Reference Interval:

Negative, Equivocal or Positive

  Reportable Disease:

Report positive Syphilis IgG within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases. 


Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

A positive T. pallidum IgG result will be confirmed by RPR with quantitation (titer) if positive.  If RPR is negative, An FTA will be performed.

A non-treponemal test, such as RPR, is recommended for children 6 months of age and younger.

NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.

  Revised: 6/21/2021