Test: West Nile Antibody IgG & IgM, CSF 
  Synonym: West Nile Virus Antibody 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Varies by season. Testing is performed on Wednesday, results reported next day by 1700. Specimen must be received by 0700 on day of testing. Days performed may increase with seasonal demand for the test. Off season testing is within 14 days of specimen receipt in lab. 
  Specimen: CSF 
  Collection Device: Sterile, capped tube.
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms 
  Volume: 1.0 mL 
  Storage/Transport: Prior to and during shipment: Refrigerate 2-8°C; if transport to the lab is not the same day as collection, frozen specimens are recommended

Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped with frozen gel packs. Frozen specimens should be shipped on Dry Ice. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org 
  Unacceptable: Specimen with repeated freeze/thaw cycles 
  Specimen Stability: Ambient: =8 hrs; Refrigerated: 48 hrs; Frozen: 1 month  
  Reference Interval: IgG: Negative
IgM: Negative 
  Reportable Disease: Positives will be reported to local or state health department.
Refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send documented approval to NPHL@unmc.edu or call (402) 559-9444.

The Nebraska State Epidemiologist does not recommend screening for only West Nile IgG antibody. All requests for only IgG will be sent to a reference laboratory and will be charged to the submitter. 
  Revised: 7/11/2019