| Test: | HIV Antigen/Antibody Panel |
| Synonym: | HIV ELISA screen, HIV screen |
| Method: | Multiplex flow immunoassay |
| Availability: | Daily, results in 24 hours |
| Specimen: | Blood |
| Collection Device: | 6 ml SST (Gold) tube, 6ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube |
| Volume: | Minimum 0.5 ml serum or plasma |
| Storage/Transport: | Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube. |
| Unacceptable: | Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours |
| Specimen Stability: | After separation from cells: ambient if < 24 hours; refrigerated if > 24 hours up to 14 days; frozen if > 14 days. |
| Reference Interval: | Nonreactive |
| Reportable Disease: | Reactive is reportable |
| Comments: | Reflexed to HIV 1,2 Antibody confirmation testing if antigen/antibody screen is reactive. Detection of HIV-1 p 24 antigen in the absence of detectable HIV antibody may indicate an acute infection. Confirmation testing is performed using the quantitative HIV RNA assay. All HIV-2 antibody reactive will be confirmed by the CDC. |
| Revised: | 2/28/2017 |