| Test: | HIV 1,2 Antibody Confirmation EIA |
| Synonym: | HIV confirmation, HIV Monospot |
| Method: | Rapid enzyme immunoassay |
| Availability: | Monday - Friday, within 72 hours of positive HIV Antigen/Antibody screen |
| Specimen: | Blood |
| Collection Device: | 6 ml SST (Gold) tube, 6 ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube |
| Volume: | Minimum 0.5 ml serum or plasma |
| Storage/Transport: | Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube. |
| Unacceptable: | Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours |
| Specimen Stability: | Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours |
| Reference Interval: | Nonreactive |
| Reportable Disease: | Reactive is reportable |
| Comments: | Indeterminate results are reflex tested by the HIV RNA assay. All HIV 2 reactive results will be confirmed by the CDC. |
| Revised: | 2/28/2017 |