Test: Shigella Serotyping 
  Synonym: Shigella typing, serogrouping 
  Method: Latex Agglutination 
  Availability: Test performed Daily, results within 24 - 48 hours. 
  Specimen: Pure isolate on media slant or plate 
  Collection Device: Organisms isolated from routine cultures submitted on media slant
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms 
  Volume: Not applicable 
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org 
  Unacceptable: Mixed cultures 
  Specimen Stability: Stable at Ambient 15-25°C 
  Reference Interval: Negative 
  Reportable Disease: Positive results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
Supplemental Forms Required – Nulirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.

Test Includes serogrouping and banking of referred organism. Include the test method used for identification on the request form. 
  Revised: 7/15/2019