Test:

Mycobacterium tuberculosis Complex Genotyping
Supplemental Forms Required (only if not entered into NUlirt) –  
Mycobacterium tuberculosis (MTB) Supplemental Form

NUlirt packing list or manual NPHL Test Order Form (completed in entirety) must accompany the specimen.

 
  Synonym:

Spoligotyping, Genotyping MTB

 
  Method:

Whole genome sequencing

 
  Availability:

Test performed Monday – Friday

 
  Specimen:

Pure isolate of M. tuberculosis complex in liquid (MGIT) or solid media (LJ or Middlebrooks).

 
  Collection Device:

Pure isolate on media slant or plate, sealed with parafilm or tape
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms

 
  Volume:

Submitting lab should keep 1mL of MGIT broth in their laboratory and send remainder or a subculture on solid media.

 
  Storage/Transport:

Prior to and during shipment: Ambient 15-25°C 

Shipping instructions, including specimen-handling requirements during transport:  Sender must contact the NPHL by email or phone before shipping.
All screw-top tubes must be sealed with parafilm or tape.  Vacutainer tubes do not require parafilm or tape to seal,
All isolates are Category A and must be packaged UN 2814 Infectious Substances, affecting humans (Category A) and shipped via FedEx.  Omaha & Lincoln laboratories can ship via NPHL ground courier by calling client services (866) 290-1406 and request exclusive Category A courier.
See instructions and shipping address: http://nphl.org/

 
  Unacceptable:

Mixed isolates

 
  Specimen Stability:

Stable at Ambient 15-25°C

 
  Reference Interval:

Not applicable

 
  Reportable Disease:

Positive MTB complex results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.

 
  Comments:

Results will be reported to the submitting laboratory and the state TB director. 

Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

 
  Revised: 1/8/2020