Mycobacterium tuberculosis Complex Genotyping
Supplemental Forms Required (only if not entered into NUlirt) –
Mycobacterium tuberculosis (MTB) Supplemental Form

NUlirt packing list or manual NPHL Test Order Form (completed in entirety) must accompany the specimen.


Spoligotyping, Genotyping MTB


Whole genome sequencing


Test performed Monday – Friday


Pure isolate of M. tuberculosis complex in liquid (MGIT) or solid media (LJ or Middlebrooks).

  Collection Device:

Pure isolate on media slant or plate, sealed with parafilm or tape
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms


Submitting lab should keep 1mL of MGIT broth in their laboratory and send remainder or a subculture on solid media.


Prior to and during shipment: Ambient 15-25°C 

Shipping instructions, including specimen-handling requirements during transport:  Sender must contact the NPHL by email or phone before shipping.
All screw-top tubes must be sealed with parafilm or tape.  Vacutainer tubes do not require parafilm or tape to seal,
All isolates are Category A and must be packaged UN 2814 Infectious Substances, affecting humans (Category A) and shipped via FedEx.  Omaha & Lincoln laboratories can ship via NPHL ground courier by calling client services (866) 290-1406 and request exclusive Category A courier.
See instructions and shipping address: http://nphl.org/


Mixed isolates

  Specimen Stability:

Stable at Ambient 15-25°C

  Reference Interval:

Not applicable

  Reportable Disease:

Positive MTB complex results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.


Results will be reported to the submitting laboratory and the state TB director. 

Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

  Revised: 1/8/2020