Chikungunya Virus RNA Detection


Arbovirus, Chikungunya virus RT-PCR


RT-Polymerase Chain Reaction (PCR)




Serum and cerebrospinal fluid (CSF)

  Collection Device:

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms




Prior to and during shipment:  Frozen on Dry Ice.
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Ship in insulated box on dry ice using UN1845 label. Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.


Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles. Heparinized specimens.

  Specimen Stability:

2-8°C for 72 h, if > 3 d, freeze

  Reference Interval:

NEGATIVE - No significant level of detectable chikungunya virus RNA
POSITIVE – Chikungunya virus RNA detected

  Reportable Disease:

 A positive result is reportable as Arbovirus infection under Title 173, report within 7 days


Pre-approval needed from the state or local health department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

Test Request Order Form must accompany the specimen to include the following information:  symptoms and date of onset, pregnancy status, travel and vaccine history.

  Revised: 3/15/2021