Legionella pneumophila Antigen, Urine
Order in NUlirt or complete NPHL Test Order Form


Leigonnaires' disease or LD; Legionellosis; Pontiac Fever


Immunochromatographic assay


Test performed daily, reported within 24 hours.  Clients are notified of positive results



  Collection Device:

Sterile leak-proof container. 
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms


5mL Random Urine    Minimum:  0.5mL


Prior to and during shipment:  Refrigerate 2-8°C.  
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material 
Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.


Specimens in preservatives

  Specimen Stability:

Stable at Refrigerated 2-8°C for up to 14d.

  Reference Interval:

Negative for Legionella pneumophila Antigen

  Reportable Disease:

Report positive Legionella pneumophila Antigen within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases


Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

This assay detects Legionella pneumophila serogroup one (1) antigen.  A negative test result does not rule out the possibility of Legionella infection due to other serogroups or species of Legionella.  A positive result may indicate a recent or remote infection with serogroup 1. 

A respiratory culture should be considered for outbreak investigational purposes. All positive Legionella isolated should be submitted to the NPHL for molecular sequencing to supplement and epidemiological investigation. 

NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.

  Revised: 6/11/2021