Zika Virus Antibody IgM


Arbovirus, Arbo-Serology, Zika virus serology




Test performed Monday-Friday; Turn-around time 7 days


Whole blood

  Collection Device:

Clot (Red) or SST(Gold) tube
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and order forms


Serum: 1.0 mL; Minimum: 0.25 mL


Centrifuge and aliquot serum into sterile leak proof tube, within 2 hours of collection.

Prior to and during shipment:  Store serum at 2-8°C.

Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped with frozen gel-packs.  Transport via NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org/


Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; samples that have undergone multiple freeze thaw cycles

  Specimen Stability:

2-8°C for 7 days, if >7 days, ≤-20°C. Avoid repeated freeze-thaw cycles.

  Reference Interval:

Negative - No significant level of detectable Zika IgM antibody.
Positive - IgM antibody to Zika virus detected, reflexed for confirmation testing

  Reportable Disease:

A positive result is reportable as Arbovirus infection under Title 173, report within 7 days


Pre-approval needed from state or local health department. Send documented approval to NPHL@unmc.edu or call (402) 559-9444.

Use for patients whose symptoms began, or whose documented exposure occurred, at least 14 days prior to testing. Should also be used as follow-up for patients with negative serum and urine results from molecular testing performed ?14 days after symptom onset.

Substantial serological cross-reactivity exists among the flaviviruses (Dengue virus, St. Louis encephalitis virus and West Nile Virus) and current IgM antibody assays cannot reliable distinguish among them. Therefore an IgM positive result in a Zika IgM ELISA test will be reflexed for confirmation testing using RT-PCR and/or plaque-reduction neutralization testing which will result in a delay in the reporting of final results.

Molecular detection of Zika virus RNA can be performed on specimens collected after 7days of symptom onset. Refer to the Zika Virus RNA Detection (link to actual page) test directory information.

For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.

  Revised: 2/28/2021