Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Syphilis IgG  
  Synonym: Treponema pallidum IgG 
  Method: Enzyme-Linked Immunosorbent Assay  
  Availability: Monday-Friday by 0800; results same day  
  Specimen: Blood  
  Collection Device: 6.0 mL Gold (SST) tube  
  Volume: 2.0 mL serum (Min: 0.5 mL)  
  Storage/Transport: Refrigerate; if transport to lab is not the same day as collected, aliquot the serum into clean tube  
  Unacceptable: Plasma or other body fluids, severely lipemic, contaminated, or hemolyzed specimens  
  Specimen Stability: After separation from cell, 48 hours refrigerated and 1 year frozen; avoid multiple freeze/thaw cycles  
  Reference Interval: Negative  
  Reportable Disease:  
  Revised: 7/7/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.