Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Syphilis IgG 
  Synonym: Treponema pallidum IgG 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Monday-Friday by 0800; results same day 
  Specimen: Blood 
  Collection Device: 6.0 mL Gold (SST) tube 
  Volume: 2.0 mL serum (Min: 0.5 mL) 
  Storage/Transport: Refrigerate; if transport to lab is not the same day as collected, aliquot the serum into clean tube 
  Unacceptable: Plasma or other body fluids, severely lipemic, contaminated, or hemolyzed specimens 
  Specimen Stability: After separation from cell, 48 hours refrigerated and 1 year frozen; avoid multiple freeze/thaw cycles 
  Reference Interval: Negative 
  Reportable Disease:  
  Revised: 7/7/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.