Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Culture, Aerobic with Gram Stain 
  Synonym: C&S, Aerobic Culture 
  Method: Standard reference procedures for gram stain, aerobic culture, and identification of potential pathogen; susceptibility performed when indicated 
  Availability: Daily; gram stain results same day, preliminary results at 1 day, and final results at 3 days 
  Specimen: Skin, ear, nasopharyngeal, wound, placenta, vaginal, cervical 
  Collection Device: Sterile container or two culturette swabs labeled with source; use enough sterile saline to moisten the tissue if using a sterile container 
  Volume: No minimum 
  Storage/Transport: Ambient; transport to lab same day as collected 
  Specimen Stability: Specimen stable at ambient but should be sent to lab ASAP 
  Reference Interval: No growth 
  Reportable Disease:  
  Comments: Label specimen container with patient name, date of birth and source of specimen. 
  Revised: 7/7/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.