Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Cryptosporidium / Giardia Antigen Screen 
  Synonym: O & P 
  Method: Enzymatic Immunoassay 
  Availability: Monday, Wednesday, Friday by 0900; results same day 
  Specimen: Stool 
  Collection Device: Fresh stool in sterile container or if transport to lab > 2 hours, stool in Proto-fix vial for preservation. Add stool to fill line marked on Proto-fix vial, seal and shake vigorously. Add bloody or watery portions of stool if present. 
  Volume: Walnut size stool or 10 mL of stool in preservative 
  Storage/Transport: Ambient; transport to lab as soon as possible 
  Unacceptable: Samples in PVA and formalin, dry specimens, leaking containers, specimens contaminated with oil, barium or urine, rectal swabs, multiple specimens per day 
  Specimen Stability: Preserved stool stable for 7 days 
  Reference Interval: Negative 
  Reportable Disease:  
  Comments: Test includes: examination of stool for the presence of Giardia and Cryptosporidium by EIA assay. If the patient has had foreign travel or continued symptoms and tested negative by antigen screen then recommend ordering Ova & Parasite, Complete, Foreign Travel Only. If Cyclospora/Isospora is suspected, order Ova & Parasites, Stool for Cyclospora & Isospora Stain. One Proto-fix vial can be submitted for all Ova & Parasite tests except Pinworm. If pinworm is suspected, a scotch tape preparation or pinworm collection paddle should be submitted to the lab instead of stool. Preservative available from Regional Pathology Services. 
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.