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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: HIV Antigen/Antibody Panel 
  Synonym: HIV ELISA screen, HIV screen 
  Method: Multiplex flow immunoassay 
  Availability: Daily, results in 24 hours 
  Specimen: Blood 
  Collection Device: 6 ml SST (Gold) tube, 6ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube 
  Volume: Minimum 0.5 ml serum or plasma 
  Storage/Transport: Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube. 
  Unacceptable: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours 
  Specimen Stability: After separation from cells: ambient if < 24 hours; refrigerated if > 24 hours up to 14 days; frozen if > 14 days. 
  Reference Interval: Nonreactive 
  Reportable Disease: Reactive is reportable 
  Comments: Reflexed to HIV 1,2 Antibody confirmation testing if antigen/antibody screen is reactive.

Detection of HIV-1 p 24 antigen in the absence of detectable HIV antibody may indicate an acute infection. Confirmation testing is performed using the quantitative HIV RNA assay.

All HIV-2 antibody reactive will be confirmed by the CDC. 
  Revised: 2/28/2017 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.