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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Measles Virus Antibody, IgM 
  Synonym: Measles Virus Serology, Rubeola Antibody 
  Method: CDC capture IgM, Commercial indirect IgG 
  Availability: Monday - Friday. Turn-around time 2-7 days 
  Specimen: Blood 
  Collection Device: One 6.0 mL SST (Gold) tube or one 6.0 mL Clot (Red) tube 
  Volume: 0.5 - 2.0 mL serum 
  Storage/Transport: Separate serum ASAP and transfer to clean tube. Refrigerate at 2-8°C. Sender should contact the NPHL by email or phone before shipping. Ship specimen Monday -Thursday overnight to avoid weekend deliveries. Refrigerated specimen should be shipped on cold packs. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address at 
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles, specimens stored at <-40°C 
  Specimen Stability: After separation from cells: ambient, < 8 hours; refrigerated (2-8°C), 72 hours; frozen (≥ -40°C), 1 year 
  Reference Interval: NEGATIVE - No significant level of detectable IgM antibody to measles virus.

EQUIVOCAL - Borderline levels of IgM antibody to measles virus. (Repeat testing in 10-14 days recommended).

POSITIVE - IgM antibody to measles virus detected 
  Reportable Disease: A positive result is reportable 
  Comments: IgM positive may not occur until 4 days post-rash onset. While the presence of IgM antibodies suggests current or recent measles infection, low levels of IgM antibodies may occasionally persist for > 12 months post-infection or immunization. Serum specimens WILL NOT be accepted for serology testing without the simultaneous submission of a specimen appropriate for Measles PCR. Test performed at CDC.

Pre-Approval needed from state or local health department. Send approval to or call (402) 559-9444
  Revised: 2/24/2015 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.