Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
 
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

 BACK | PRINT

  Test: Chlamydia trachomatis / Neisseria gonorrhoeae Amplified Probe  
  Synonym: STD PCR assay, Chlamydia/GC Nucleic acid amplification test (NAAT) 
  Method: Qualitative Target Amplification Nucleic Acid Probe (GenProbe APTIMA®)  
  Availability: Test performed Monday – Friday 
  Specimen: Male urethral, endocervical, rectal, throat, urine, and vaginal
Collection kits available from NPHL Client Services  
  Collection Device: Male urethral, endocervical, rectal, and throat use the APTIMA Unisex Swab Specimen Collection Kit; vaginal use the APTIMA Vaginal Swab Specimen Collection Kit; urine use the APTIMA Urine Specimen Collection Kit for male and female.

Collection:
Urethral male - (Discard large cleaning swab - not needed) Insert the specimen collection swab (blue shaft) 2-4 cm into the urethra. Rotate for 2-3 seconds. Immediately place swab into transport tube. Carefully break the swab shaft at the score line. Recap and Label.

Endocervical - With large cleaning swab (white shaft) remove excess mucus and blood and discard. Insert the specimen collection swab (blue shaft) into the cervical canal and rotate for 10-30 seconds. Immediately place swab into transport tube. Carefully break the swab shaft at the score line. Recap and Label.

Rectal swab - (Discard large cleaning swab - not needed) Insert the specimen collection swab (blue shaft) into the anal canal and rotate for 15-30 seconds. Immediately place swab into transport tube. Carefully break the swab shaft at the score line. Recap and Label.

Throat swab - Discard large cleaning swab - not needed) Using the specimen collection swab (blue shaft) instruct patient to tilt head backwards, open mouth and say "ah." A tongue depressor may be used to depress the tongue and facilitate visualization of pharynx. Insert swab without touching lips, teeth, tongue, or cheeks. Gently and quickly swab the tonsillar area side to side, making contact with inflamed or purulent sites. Immediately place swab into transport tube. Carefully break the swab shaft at the score line. Recap and Label.

URINE – Use APTIMA® Urine Specimen Collection Kit for Male and Female Urine Specimens: Patient should NOT urinate for at least 1 hour prior to collection of specimen. Patient should collect the 20-30 ml of the initial urine stream in a sterile preservative-free collection cup. Transfer 2 mL of urine into the urine specimen transport tube within 24 hours of collection using pipette provided. Urine level should fall between the black fill lines on the transport tube label. Make sure cap is tightly secured to eliminate leaking. Recap and Label.

Vaginal swab - Use APTIMA® Vaginal Swab Specimen Collection Kit: Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the score line. Insert the specimen collection swab (pink shaft) into the vagina about two inches past the introitus and rotate for 10-30 seconds making sure swab touches the walls of the vagina. Immediately place swab into transport tube. Carefully break the swab shaft at the score line. Recap and Label.

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: 1 swab or 20 - 30 mL urine  
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C up to 30 days
Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org 
  Unacceptable: Use only APTIMA® swab collection Specimen Collection Kits. Unacceptable specimens include wooden swabs, eyes and swab specimens without a swab in transport media. Large white swab is for preparatory cleaning of the endocervix and is unacceptable for testing. Specimens stored at ambient or refrigerator temperatures past the stability date.  
  Specimen Stability: In APTIMA® transport media: Ambient: 30 days; Refrigerated: 30 days; Frozen: 90 days. Unpreserved urine specimens are stable at ambient for 24 hours.  
  Reference Interval: Negative for C. trachomatis / N. gonorrhoeae
Positive for C. trachomatis / N. gonorrhoeae
Positive for N. gonorrhoeae  
  Reportable Disease: Positive for C. trachomatis or N. gonorrhoeae should be reported within 7 days to local or state health department.  
  Comments: A negative result does not preclude the presence of a C. trachomatis or N. gonorrhoeae infection because results are dependent on adequate specimen collection, absence of inhibitors, and sufficient rRNA to be detected. Test results may be affected by improper specimen collection, improper specimen storage, technical error, or specimen mix-up.

The positive predictive value of the test varies depending on the organism prevalence in the population, the gender of the patient and the specimen type tested. According to CDC guidelines, positive screening tests should be considered presumptive evidence of infection. Test results are reported as positive, negative or indeterminate.

If an indeterminate result is reported then submit a new specimen if indicated or treat patient based on clinical judgment.

Culture is recommended as the standard for detection in suspected sexual abuse and for suspected failure of therapy.  
  Revised: 12/18/2018 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


 BACK