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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: West Nile Virus RNA Detection, Blood 
  Method: Real-Time DNA Amplification 
  Availability: Daily 
  Specimen: Blood 
  Collection Device: 5.0 mL EDTA (Lavender) tube 
  Volume: 0.5 mL plasma 
  Storage/Transport: Refrigerate; centrifuge ASAP and transfer to clean tube 
  Unacceptable: Heparinized or grossly hemolyzed 
  Specimen Stability:  
  Reference Interval: Negative; Virus not detected 
  Reportable Disease:  
  Comments: This test was designed to evaluate a select patient population in the acute phase of disease prior to the production of antibody and for those patients whose ability to develop an antibody response may be compromised due to underlying health conditions. Clinical studies have demonstrated that virus is no longer detectable after two days of symptoms. However, after three days of becoming symptomatic, IgM can be detected in the CSF of the majority of patients. This test detects RNA from West Nile virus but may also cross-react with other flaviviruses, including St. Louis Encephalitis virus. 
  Revised: 7/6/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.