Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Legionella pneumophila Antigen, Urine  
  Synonym: Leigonnaires' disease or LD; Legionellosis; Pontiac Fever 
  Method: Immunochromatographic assay  
  Availability: NPHL Special Microbiology Requisition Required. Test performed daily with results within 24 hours. Clients are notified of positive results.  
  Specimen: Urine  
  Collection Device: Sterile leak-proof container

Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms.  
  Volume: 5.0 mL; minimum 0.5 mL  
  Storage/Transport: Prior to shipment: store refrigerated at 2 - 8 °C.

Shipping instructions, including specimen handling requirements during transport: Ship specimen Monday - Friday, in biohazard bag with absorbent material. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address: Transport via NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.  
  Unacceptable: Specimens in preservatives  
  Specimen Stability: Stable refrigerated for up to 14 days  
  Reference Interval: Negative  
  Reportable Disease: A positive test is reportable within 7 days. 
  Comments: This assay detects Legionella pneumophila serogroup one (1) antigen. A negative test result does not rule out the possibility of Legionella infection due to other serogroups or species of Legionella. A positive result may indicate a recent or remote infection with serogroup 1. 
  Revised: 7/11/2017 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.