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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Legionella pneumophila Antigen, Urine 
  Synonym: Leigonnaires' disease or LD; Legionellosis; Pontiac Fever 
  Method: Immunochromatographic assay 
  Availability: NPHL Special Microbiology Requisition Required. Test performed daily with results within 24 hours. Clients are notified of positive results. 
  Specimen: Urine 
  Collection Device: Sterile leak-proof container

Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms. 
  Volume: 5.0 mL; minimum 0.5 mL 
  Storage/Transport: Prior to shipment: store refrigerated at 2 - 8 °C.

Shipping instructions, including specimen handling requirements during transport: Ship specimen Monday - Friday, in biohazard bag with absorbent material. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address: Transport via NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup. 
  Unacceptable: Specimens in preservatives 
  Specimen Stability: Stable refrigerated for up to 14 days 
  Reference Interval: Negative 
  Reportable Disease: A positive test is reportable within 7 days. 
  Comments: This assay detects Legionella pneumophila serogroup one (1) antigen. A negative test result does not rule out the possibility of Legionella infection due to other serogroups or species of Legionella. A positive result may indicate a recent or remote infection with serogroup 1. 
  Revised: 7/11/2017 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.