Gonorrhea Culture
Order in NUlirt or complete NPHL Test Order Form


GC Screen, Gonococcal Culture, GC Screen


Standard reference procedure for Neisseria gonorrhoeae culture and identification.


Test performed Daily.  Preliminary report as soon as found positive, final for negative results at 3 days.


Any site or fluid, urethral swab, endocervical swab, rectal swab or throat swab.

  Collection Device:

JEMBEC System of Modified Thayer Martin agar sealed in a plastic bag with a CO2 generating tablet, follow package insert directions.   Note specific site on specimen and test request form. 
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms




Prior to and during shipment: Ambient 15-25°C; if any delay in transport, incubate at 37°C.
Shipping instructions, including specimen-handling requirements during transport:  Seal with Parafilm™ or tape and place in biohazard bag with absorbent material.
Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container.  Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.


Delayed transport of the plates in non-CO2 environment. Refrigerated or frozen specimens

  Specimen Stability:

Stable at Incubator Temperature 35-37°C

  Reference Interval:

Negative for Neisseria gonorrhoeae

  Reportable Disease:

Report positive Neisseria gonorrhoeae within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases


Specimens should be collected prior to the initiation of antimicrobial therapy. Transport immediately at room temperature. If there is any delay in transport, incubate at 37C.  Culture for N. gonorrhoeae is the method of choice in cases of treatment failure and sexual abuse and for non-genital sources. Media and probes are available from client services.

Antimicrobial susceptibility testing performed on all non-genital specimens and genital specimens upon request.


  Revised: 6/9/2021